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3.2 ICI resistance Despite the highly immunogenic nature of dMMR–MSI-H CRC, a clinically meaningful subset of tumors exhibits primary resistance ( Table 2 ), while others develop acquired resistance after an initial response, highlighting that TMB alone is insufficient to guarantee effective immune control ( Figure 3 )

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Human thyroid cancer cell lines Human PTC cell lines K1 and TPC-1 (Sigma-Aldrich, St