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The membranes were incubated in 1:1000 rabbit anti-GLYR1 antiserum in the blocking buffer overnight at 4 °C, washed multiple times with TBST, incubated in 1:10,000 goat anti-rabbit IgG Horseradish Peroxidase (HRP) secondary antibody (PhytoAB Inc.) in TBST for 2 h, washed again with TBST, and then developed with the SuperSignal West Pico PLUS Chemiluminescent Substrate kit (Thermo Scientific)

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