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Regarding lysozyme quantification in the cell lysate, 100,000 electroporated cells with Dlyso were washed with cold PBS, and lyzed on ice for 10 min with 100 μL lysis buffer (containing protease inhibitor cocktail)
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Increased levels of iron have been confirmed in the PD midbrain, which in turn results in iron-induced oxidative stress and neurodegeneration (Chinta and Andresen 2008)
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