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Pull-down assay Five micrograms of the bait protein c-MET927-TSI3-His was immobilized to TSE resin by incubating for 30 min on ice and washed with binding buffer PBS (Gibco) supplemented with10 Β΅M Tinzaparin and 2 mM CaCl2
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The thumb subdomain is composed of four Ξ±-helices, whereas the palm is formed by a six-stranded Ξ²-sheet
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