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While Ishikawa and Ali-Osman [14] initially reported that formation of Pt(GS) 2 complex reached a maximal level after 12 hrs in L1210 cells treated with 20 μ M CDDP, corresponding to ~60% of the intracellular Pt content, however, Berners-Price and Kuchel studied the reaction of CDDP with GSH inside intact red blood cells using 1 H spin-echo nuclear magnetic resonance (NMR) and detected no formation of Pt-GSH bonds within 4 hrs of incubation [26]

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