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Multivitamins and metformin induced b12 deficiency | PPTX
Size: How many subjects, and is there a control group
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The resulting pcDNA5FRT-GFP-H1.4 constructs (encoding the WT, RS, or RSΔC variants of H1.4) were integrated into U2OS-FlpIn cell line (Thermo Fisher) according to the manufacturer’s recommendations and GFP-positive cells were sorted three days later using a BD FACSDiscover™ S8 Cell Sorter
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