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Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-κB p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

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The peptide selectively targets cells that have already undergone senescence as a consequence of earlier chemotherapy exposure, demonstrating a favorable safety profile for potential clinical application