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01 Component-resolved HPLC and LC-MS assays separating and quantifying each of the three materials 02 Cell-free NNMT inhibition assays with IC50 and Ki determined by LC-MS/MS 03 Surface plasmon resonance and pull-down binding assays against isolated enzyme preparations 04 Spectrophotometric and LC-MS enzyme assays following NAD+ substrate turnover 05 Reference standard set for multi-analyte chromatographic method development and calibration 06 Blend stability and solubility characterisation, tracking each component separately by HPLC across freeze-thaw cycling Primary literature relevant to this listing is component-specific: quinolinium salt synthesis and NNMT enzymology for 5-Amino-1MQ, open-reading-frame identification and enzyme-binding chemistry for MOTS-c, and redox couple and cofactor enzymology for NAD+
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