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Precursor mass error of 15 ppm using monoisotopic mass was used for parent ion identifications and a 0.05 Da for fragment ions masses
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meanwhile, endogenous itaconate directly modifies IRAK4 at cysteine 13, blocking its autophosphorylation and promoting NF-κB degradation, whereas loss of itaconate leads to hypersecretion of inflammatory cytokines
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