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Approximately 100 μg of GST‐Vector or GST‐CmSWNa was incubated with His control, or GST‐Vector or GST‐CmSWNa was incubated with His‐CmFDa in a 1 mL binding buffer system (50 mM Tris pH 7.5, 100 mM NaCl, 0.25% Triton X‐100, 35 mM β‐ME) for 4 h at 4 °C

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Although the cataractous material samples procured in this study were only briefly exposed to sonication, as the overall effective phacoemulsification time in the hands of experienced surgeons typically ranges between 15–30 seconds or less, the potential impact of phacoemulsification towards induction of protein aggregation cannot be excluded