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The chromatographic gradient was performed at 400 µL/min using buffer A (10 mM ammonium formate, 0.1% formic acid in acetonitrile:water, 60:40, v/v) and buffer B (10 mM ammonium formate, 0.1% formic acid in isopropanol:acetonitrile, 90:10, v/v) as follows: 0–0.5 min, 45–48% B

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Over-supplementation is rarely harmful, but excessive dosing may cause imbalance with other B vitamins

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